Biosecurity verification in synthetic biology is often treated as an afterthought. With modern DNA synthesis pipelines accelerating worldwide, validating genetic sequence provenance and detecting hazardous biological threats in real-time is critical[cite: 13, 14].
To solve this, I built GeneSign — a zero-drift wobble codon DNA watermarking platform and biosecurity firewall with integrated AI threat rationale[cite: 3, 13].
🧬 What GeneSign Does
Zero-Drift Wobble Steganography: Injects tamper-proof cryptographic metadata into protein coding sequences (CDS) by utilizing degenerate synonymous codons without modifying amino acid translation ($\Delta GC = 0.000%$)[cite: 13]. #
Origin Provenance & Verification: Embeds dual-layer Ed25519 signatures and tracks authorization records in a tamper-resistant audit ledger[cite: 13]. #
Real-time Threat Radar: Automatically flags regulated pathogens and select agents using high-throughput biological sequence screening[cite: 13, 14]. #
NVIDIA Nemotron AI Rationale: Synthesizes regulatory guidance, biohazard threat rationales, and enforcement actions via LLM inference[cite: 3, 14]. #
1-Base Tamper Simulation: Validates hardware interlocks by illustrating instant signature invalidation under single nucleotide substitutions[cite: 13].
🛠️ Architecture & Tech Stack
Core Engine & API: Python, FastAPI, SQLite ledger, and Uvicorn. #
AI Intelligence: NVIDIA Nemotron via OpenRouter for biosecurity compliance and threat analysis[cite: 3, 7]. #
Interactive Frontend: Dark tactical HUD with Three.js-powered 3D DNA helix visualization[cite: 13]. #
Cloud Infrastructure: Production-grade deployment containerized on Render[cite: 13].
🚀 Live Demo & Code
Building this system balances biological preservation constraints with low-latency cryptographic verification. GeneSign enforces origin integrity before synthetic constructs ever reach the physical synthesizer.